FIG. 3.

GFP-Gβ and GFP-Gγ are absent from plasma membranes in phlp1⁻ cells. Cells expressing GFP-Gβ or GFP-Gγ were subjected to crude fractionation by filter lysis and collection of particulate and cytosolic fractions after centrifugation in a microcentrifuge. Equal samples were used for Western blot analysis with a GFP antibody, as described in the legend for Fig. 2A. wt, wild type.