FIG. 5.

GFP-Gβ and HA-Gγ do not coimmunoprecipitate in extracts of phlp1⁻ cells. Cell lysates were prepared from nontransfected cells and from cells expressing either GFP-Gβ alone or both GFP-Gβ and HA-Gγ and immunoprecipitated with a monoclonal GFP antibody. Half of the immunoprecipitates were used for Western blot analysis with a polyclonal GFP antibody (α-GFP), and the other half were used for Western blot analysis with an antibody recognizing hemagglutinin tags (α-HA). wt, wild type.