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GFP-Gγ exhibits monomer-type diffusion characteristics in phlp1− cells. Comparison of the diffusion coefficient distribution of GFP-Gγ in wild-type AX3 (black bars), phlp1− (open bars), and gβ− (gray bars) cells. The diffusion coefficient distribution of GFP-Gγ proteins was determined in the cytosol of different cell lines by using FCS. Bars indicate the fraction of the total number of observations in which a specific diffusion coefficient (D) was found.
