FIG.2.

Effects of Ada2a and Ada2b mutations on H3 K14, H3 K9, and H4 K8 acetylation on polytene chromosomes. Chromosomes immunostained with acetylated H3- and H4-specific polyclonal antibodies (H3AcK9, H3AcK14, and H4AcK8) and a Pol II-specific monoclonal antibody (Pol II 7G5) are shown. Genotypes are indicated at the top. The pictures indicating immunostaining in different mutants were obtained with identical data-recording settings. The inset shows Ada2b and wild-type polytene chromosomes immunostained in a mixed preparation with H3AcK14-specific polyclonal antibody. Chromosomes were identified on the basis of the structure of balancer chromosomes in wild-type animals.