FIG. 4.

Effects of Ada2a and Ada2b mutations on eye pigment formation. (A) Red eye pigment contents of Ada2a/Dmp53, Ada2b/Dmp53, and Ada2b/+ adult heterozygotes. The pigment contents of groups of 10 animals with the indicated genetic backgrounds were determined as described in Materials and Methods. Ratios of pigment contents of Ada2 mutants to those of the corresponding controls (p53/Ada2a versus p53/+, p53/Ada2b versus p53/+, and +/Ada2b versus +/+) are shown. The level of rosy mRNA is reduced in Ada2b mutants, as determined by RT-PCR (B and C), quantitative PCR (D), and DNA microarray analysis (E). The levels of rosy mRNA and L17A mRNA as a control were determined by RT-PCR with total RNA samples prepared from third-instar larvae (B) and 10-h pupae (C). The genotypes are indicated. wt, wild type; gDNA, genomic DNA control. (Note that Ada2a animals do not reach the pupa stage.) Arrows point to specific products present at a decreased level. (D) Level of rosy mRNA in Ada2a and Ada2b third-instar larvae compared to that in wild-type controls, as determined by quantitative RT-PCR. (E) Levels of selected mRNAs, including rosy, scarlet, Ada2a, and Ada2b, in wild-type and Ada2b mutant white pupae determined on three pairs of microarrays.