FIG. 7.

Knock-down of CYLD by siRNA restores BT549 cell morphology and inhibits apoptosis. (A) An equal number (5 × 10⁴) of BT-BAF57, BT-BAF57-NSsiRNA, and BT-BAF57-CYLDsiRNA cells was seeded into 10-cm plates, and pictures were taken at ×40 magnification. (B) To measure the mRNA levels of CYLD, BAX, and BCL-2, RT-PCR was conducted using total RNA from the indicated stable cell lines. As a control GAPDH levels were also analyzed. (C) To measure endogenous CYLD protein levels, Western blot analysis was conducted using whole-cell extracts from the indicated stable cell lines. As a control HDAC2 levels were also analyzed. (D) An equal number (5 × 10⁴) of BT-BAF57, BT-BAF57-NSsiRNA, and BT-BAF57-CYLDsiRNA cells was seeded into 10-cm plates and analyzed by FACS analysis as described in the legend of Fig. 6D.