FIG. 1.

Colocalization of SRp38 with snRNPs during mitosis. (A) SRp38 colocalizes with snRNPs during mitosis but not during interphase of the cell cycle. HeLa cells were fixed in 4% paraformaldehyde and immunostained with an affinity-purified SRp38 polyclonal antibody (green) and the Y12 monoclonal antibody (red). Confocal optical sections of double-labeled cells were analyzed. In the left panels, a and h, phase-contrast images of the cells are shown; in the right panels, d, g, k, and n, merged images of the two antibody-stained fields are shown. Arrows indicate dots that colocalize, and arrowheads indicate dots that do not colocalize. (B) SRp38 does not colocalize with SC35 either in interphase or during mitosis. In the left panels, a and h, phase-contrast images of the cells are shown; in the right panels, d, g, k, and n, merged images of the two antibody-stained fields are shown. Arrowheads indicate dots that are not colocalized.