FIG. 2.

Colocalization of SRp38 with snRNPs in response to heat shock. (A) SRp38 colocalizes with snRNPs upon heat shock. Asynchronously grown HeLa cells were incubated at 45°C for the indicated times (e to h, 30 min; i to l, 75 min) and were fixed in 4% paraformaldehyde. Fixed cells were immunostained with an affinity-purified SRp38 polyclonal antibody (green) and the Y12 monoclonal antibody (red). In the left panels, a, e, and i, phase-contrast images of the cells are shown; in the right panels, d, h, and l, merged images of the two antibody-stained fields are shown. Arrows indicate dots that colocalize, and arrowheads indicate dots that do not colocalize. (B) SRp38 does not colocalize with SC35 upon heat shock. In the left panels, a, e, and i, phase-contrast images of the cells are shown; in the right panels, d, h, and l, merged images of the two antibody-stained fields are shown. Arrowheads indicate dots that are not colocalized.