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. 2005 Sep;25(18):8334–8343. doi: 10.1128/MCB.25.18.8334-8343.2005

FIG. 5.

FIG. 5.

Characterization of a C-terminal RS2 domain deletion mutant of SRp38. (A) Diagrams of SRp38, SRp38-2, and a C-terminal RS2 domain deletion mutant of SRp38 (SRp38ΔRS2). (B) Coomassie blue-stained SDS gel of the baculovirus-produced recombinant His-tagged proteins diagrammed in panel A. Dephosphorylated SRp38ΔRS2 (dSRp38ΔRS2, lane 3) was prepared from phosphorylated SRp38ΔRS2 (lane 2) by treatment with CIP and repurification. (C) SRp38ΔRS2 inhibits splicing at high concentrations in nuclear extract (NE; lanes 2 to 4) or S100 extract complemented with recombinant SC35 (lanes 7 to 9). (D) dSRp38ΔRS2 inhibits β-globin pre-mRNA splicing. Twenty-five to 100 ng of dSRp38ΔRS2 was added to splicing reactions with NE.