FIG. 6.

EGFP-Fbh1 foci induced by DNA-damaging agents or appearing spontaneously in the rhp51Δ mutant. (A) Cells expressing the EGFP-Fbh1 fusion protein (MPF51) without treatment, irradiated with γ rays (500 Gy), and cultured for 1 h at 30°C or treated with MMS (0.1%) for 1 h at 30°C were photographed using a fluorescence microscope. The scale bar indicates 10 μm. (B) Cells expressing the EGFP-Fbh1 fusion protein (MPF51) irradiated with γ rays (500 Gy) and cultured for 1 h at 30°C or treated with MMS (0.1%) for 1 h at 30°C were photographed using a fluorescence microscope, and the distribution of cell lengths was determined. Septated cells were classified separately. Open and solid bars indicate cells with or without Fbh1 foci, respectively. (C) rhp51Δ cells expressing the EGFP-Fbh1 fusion protein (MPF52) without or with MMS (0.1%) treatment for 1 h were photographed using a fluorescence microscope. The scale bar indicates 10 μm.