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. 2005 Sep;25(18):8139–8149. doi: 10.1128/MCB.25.18.8139-8149.2005

FIG. 5.

FIG. 5.

Overexpression of HuR stabilizes PMA-induced SLC11A1 mRNA. (A) HL-60 cells stably transfected with either plasmid pcDNA3.0-HuR-Flag or pcDAN3.0-Flag, as well as wild-type HL-60 cells, were treated with PMA for 3 days, a procedure followed by the addition of actinomycin D (Act D) (2 μg/ml), and RNA was isolated 0, 1, 2, 4, 6, and 8 h later. Northern blotting was performed by using SLC11A1 and 18S rRNA cDNA probes. (B) SLC11A1 mRNA decay curves derived from a densitometry analysis of the Northern blot hybridization. The half-life of SLC11A1 mRNA in pcDNA3-HuR-Flag-transfected cells was much longer (mean, 8.8 ± 0.4 h) than those observed in pcDNA3.0-Flag-transfected cells (mean, 4.7 ± 0.3 h) and in wild-type cells (mean, 4.1 ± 0.4 h). There was a significant difference between the half-life of SLC11A1 mRNA in the wild type and that in HuR-overexpressing cell lines (t test; P < 0.001).