FIG. 2.

Fbh1 functions in an Rhp51-dependent DNA repair pathway. (A) Spot assay comparing the genotoxin sensitivities of wild-type (MCW1221), fbh1Δ (MCW1490), rhp51Δ (MCW1088), and fbh1Δ rhp51Δ (MCW1587) strains. The plates were photographed after 5 days of incubation. (B) Detection of EYFP-Fbh1 foci by fluorescence microscopy. Strain MCW1548, which expresses EYFP-Fbh1 from plasmid pMW651, was grown at 30°C with and without 10 μM CPT for 4 h as indicated. The arrows in the right panel indicate the position of foci. (C) Colocalization of Fbh1 and Rhp51 at damage-induced foci. Foci were induced by CPT as for panel B. The strain is MCW1556, which expresses ECFP-Rhp51 from the endogenous rhp51 promoter and EYFP-Fbh1 from pMW651.