FIG. 5.
The importance of F-box and helicase activities to Fbh1's ability to inhibit DNA repair in the absence of Rad22. (A) Spot assay comparing the abilities of fbh1D485N and fbh1Δ to suppress rad22Δ genotoxin hypersensitivity. The strains are wild type (MCW1221), fbh1D485N (MCW1718), rad22Δ (MCW1285), fbh1D485N rad22Δ (MCW1767), and fbh1Δ rad22Δ (MCW1553). The plates were photographed after 5 days of incubation. (B) UV survival curves of fbh1D485N rad22Δ (MCW1767) and fbh1Δ rad22Δ (MCW1553) strains. The error bars represent the standard deviations about the mean values. (C) Abilities of wild-type and mutant Fbh1 proteins to complement the genotoxin resistance of an fbh1Δ rad22Δ strain (MCW1553). Vector refers to empty pREP41. The bottom row of each panel shows results for the rad22Δ strain (MCW1285) carrying pREP41. The medium was yeast nitrogen base, and plates were photographed after 4 days of incubation.