dGcn5 RNA interference depletes imaginal disks of acetylated H3 K9 and H3 K14 residues. The results of a confocal analysis of GFP (green) and acetylated H3 K9 (A), acetylated H3 K14 (B), acetylated H4 K8 (C), acetylated H4 K16 (D) dimethylated H3 K4 (E), or dimethylated H3 K9 (F) in wing disks from en-GAL4 UAS-GFP/UAS-IR[Gcn5] late-third-instar larvae are shown. (G) Western blot of da-GAL4/UAS-IR[Gcn5] (IR[Gcn5]) and +/UAS-IR[Gcn5] (WT) late-third-instar larvae probed with antibodies against dGcn5, H3-AcK9, H3-AcK14, and H4-AcK8, as indicated.