FIG. 6.
Complementation of histone acetylation and dGcn5 chromosome binding in dGcn5 mutants. Polytene chromosomes from Gcn5E333st/sex204 da-GAL4 third-instar mutant larvae heterozygous for the indicated transgenes were stained with an antibody directed against acetylated H3 K9 and H3 K14 residues (A, C, E, G, and I) or an antibody against the bromodomain of the dGcn5 protein (B, D, F, H, and J). A Western blot of da-GAL4 late-third-instar larvae heterozygous for the indicated dGcn5 variant transgenes (K) was probed with an antibody raised against the dGcn5 bromodomain. The asterisk indicates a degradation product of the Gcn5ΔHAT protein.