FIG. 5.

cSH2 and SH3 domains of p85α interact with Gab2. GST fusion proteins containing p85α or the indicated p85α fragments were used for the pull-down assay along with the total cell lysates isolated from (A) p210BCR/ABL-positive 32Dcl3 cells, (B) 32Dcl3 cells expressing the p190BCR/ABL wild type (wt) or indicated mutants, and (C) GFP⁺ p210BCR/ABL-positive Gab2^+/+ and Gab2^−/− cells expressing similar levels of p210BCR/ABL kinase (Lysates). The reactions were examined by Western analysis to detect Gab2 (A, upper box), BCR/ABL (B, C, upper boxes), and GST (lower boxes) proteins.