TABLE 1.
Strains used in this study
| Strain | Description | Reference |
|---|---|---|
| RN4220 | Restriction-deficient, methylation-proficient cloning intermediate | (78) |
| JE2 | USA300, wild type (WT) | (20) |
| ispA::Tn | ispA transposon from NE1447 backcrossed into WT JE2 | This study |
| hepT::Tn | hepT transposon from NE1920 backcrossed into WT JE2 | This study |
| ΔhepT | In-frame deletion of hepT constructed using pKOR1-ΔhepT | This study |
| ΔhepT ispA::Tn | ispA transposon from NE1447 transduced into ΔhepT | This study |
| menE::Tn | Tn917 inserted into menE backcrossed into WT JE2 | (71) |
| ΔmenB | In-frame deletion of menB constructed using pKOR1-ΔmenB | This study |
| menC::Tn | Tn917 inserted into menC backcrossed into WT JE2 | (71) |
| qoxA::Tn | qoxA transposon from NE92 backcrossed into WT JE2 | This study |
| cydA::Tn | cydA transposon from NE117 backcrossed into WT JE2 | This study |
| ΔmenB qoxA::Tn | qoxA transposon from NE92 transduced into ΔmenB | This study |
| ΔmenB cydA::Tn | cydA transposon from NE117 transduced into ΔmenB | This study |
| crtM::Tn | crtM transposon from NE2499 backcrossed into WT JE2 | This study |
| ΔhepT crtM::Tn | crtM transposon from NE2499 transduced into ΔhepT | This study |
| ΔispA | In-frame deletion of ispA constructed using pKOR1-ΔispA | This study |
| ΔispA qoxA::Tn | qoxA transposon from NE92 transduced into ΔispA | This study |
| ΔispA cydA::Tn | cydA transposon from NE117 transduced into ΔispA | This study |
| ΔhepT qoxA::Tn | qoxA transposon from NE92 transduced into ΔhepT | This study |
| ΔhepT cydA::Tn | cydA transposon from NE117 transduced into JE2 ΔhepT | This study |
| ispA::Tn suppressor 115 | Gentamicin-resistant pigmented ispA::Tn isolate that harbors the HepTA72E allele | This study |
| ispA::Tn suppressor 144 | Gentamicin-resistant pigmented ispA::Tn isolate that harbors the HepTA72E allele | This study |
| ispA::Tn suppressor 164 | Gentamicin-resistant pigmented ispA::Tn isolate that harbors the HepTA72E allele | This study |
| ispA::Tn suppressor 165 | Gentamicin-resistant nonpigmented ispA::Tn isolate that harbors the HepTA165T allele | This study |
| JE2 pOS1 | Empty pOS1 plasmid vector | This study |
| ispA::Tn pOS1 | Empty pOS1 plasmid vector | This study |
| ispA::Tn pOS1-ispA | ispA::Tn harboring a pOS1 plasmid encoding the ispA gene under the control of the Plgt promoter | This study |
| ΔhepT pOS1 | Empty pOS1 plasmid vector control | This study |
| ΔhepT pOS1-hepT | ΔhepT harboring a pOS1 plasmid encoding the hepT gene under the control of the Plgt promoter | This study |
| ΔhepT ispA::Tn pOS1 | Empty pOS1 plasmid vector control | This study |
| ΔhepT ispA::Tn pOS1-hepT | ΔhepT ispA::Tn harboring a pOS1 plasmid encoding the hepT gene under the control of the Plgt promoter | This study |
| ΔhepT ispA::Tn pOS1-ispA | ΔhepT ispA::Tn harboring a pOS1 plasmid encoding ispA under the control of the Plgt promoter | This study |
| ΔhepT ispA::Tn pOS1-hepTA72E | ΔhepT ispA::Tn harboring a pOS1 plasmid encoding the hepTA72E allele from ispA::Tn suppressor 115 under the control of the Plgt promoter | This study |