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. 2025 Jul 21;16(8):e00845-25. doi: 10.1128/mbio.00845-25

Fig 1.

Cochliobolus heterostrophus ΔChcpc1 mutant shows white colonies, no conidiation, minimal melanin, reduced melanin-gene expression, and decreased maize virulence compared to wild-type and complemented strains with dark phenotypes.

ChCpc1 was required for conidiation, melanin production, and virulence in C. heterostrophus. (A) Morphology of indicated strains on CMX agar plates and liquid cultures. (B) Statistical analysis of the colony diameter of indicated strains grown on CMX for 5 days. (C) Conidiation of indicated strains. (D) Statistical analysis of the intracellular melanin content of indicated strains. (E) Relative expression levels of melanin biosynthesis-related genes in WT, Δcpc1 mutant, and complemented strains. (F) Virulence of WT, Δcpc1 mutant, and complemented strains on detached maize leaves at 2 and 3 days post-inoculation (dpi). (G) Statistical analysis of the lesion area of indicated strains at 2 and 3 dpi. GraphPad Prism program’s t-tests and multiple t-tests were used for the analysis of significant differences. The bars indicate the standard error of means of three replications.