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. 2025 Jul 30;16:1614492. doi: 10.3389/fmicb.2025.1614492

Table 8.

Top 30 pathways identified by PICRUSt2.

Overall Buchnera All other genera
Pathway Count Pathway Count Pathway Count
Fatty acid beta-oxidation I 49990374.39 Fatty acid beta-oxidation I 47863170.30 Aerobic respiration I (cytochrome C) 4020259.41
Anhydromuropeptides recycling 43950267.23 Anhydromuropeptides recycling 42417511.74 Pyruvate fermentation to isobutanol (engineered) 2772569.39
TCA cycle I (prokaryotic) 43150337.13 TCA cycle I (prokaryotic) 41048308.93 Gondoate biosynthesis (anaerobic) 2763286.94
Glycogen degradation I (bacterial) 41334843.09 Glycogen degradation I (bacterial) 39880951.18 Mycolate biosynthesis 2430000.10
Pentose phosphate pathway (non-oxidative branch) 40209945.56 Pentose phosphate pathway (non-oxidative branch) 38368121.19 L-isoleucine biosynthesis II 2422960.26
L-isoleucine biosynthesis II 39849625.83 L-isoleucine biosynthesis II 37428656.17 Fatty acid elongation—saturated 2396467.81
Mixed acid fermentation 38199142.29 Mixed acid fermentation 36682065.34 L-valine biosynthesis 2313292.91
Pyruvate fermentation to isobutanol (engineered) 38100324.98 Superpathway of L-alanine biosynthesis 35986568.69 L-isoleucine biosynthesis I (from threonine) 2313292.91
L-valine biosynthesis 37781648.12 Peptidoglycan maturation (meso-diaminopimelate containing) 35935247.60 Oleate biosynthesis IV (anaerobic) 2258649.33
L-isoleucine biosynthesis I (from threonine) 37781648.12 TCA cycle VII (acetate-producers) 35921454.54 Fatty acid beta-oxidation I 2243395.81
Superpathway of L-alanine biosynthesis 37772950.94 Pyruvate fermentation to isobutanol (engineered) 35526994.20 Cis-vaccenate biosynthesis 2222135.39
TCA cycle VII (acetate-producers) 37632344.03 L-valine biosynthesis 35476963.41 Palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) 2194399.64
Peptidoglycan maturation (meso-diaminopimelate containing) 37561343.79 L-isoleucine biosynthesis I (from threonine) 35476963.41 Stearate biosynthesis II (bacteria and plants) 2148106.46
Superpathway of branched amino acid biosynthesis 36744146.58 Superpathway of branched amino acid biosynthesis 34714338.68 TCA cycle I (prokaryotic) 2140322.97
Sulfate reduction I (assimilatory) 35835117.59 Sulfate reduction I (assimilatory) 33815384.14 (5Z)-dodec-5-enoate biosynthesis 2139672.10
Superpathway of phospholipid biosynthesis I (bacteria) 34688494.38 Superpathway of phospholipid biosynthesis I (bacteria) 32695985.67 Superpathway of fatty acid biosynthesis initiation (E. coli) 2121814.72
Superpathway of adenosine nucleotides de novo biosynthesis I 34337541.32 Starch degradation V 32652900.69 Superpathway of L-serine and glycine biosynthesis I 2114370.67
L-isoleucine biosynthesis III 34249341.67 Superpathway of adenosine nucleotides de novo biosynthesis I 32550201.50 CDP-diacylglycerol biosynthesis I 2100599.12
Starch degradation V 34051427.17 L-isoleucine biosynthesis III 32366604.71 CDP-diacylglycerol biosynthesis II 2100599.12
Guanosine deoxyribonucleotides de novo biosynthesis II 33696318.85 Guanosine deoxyribonucleotides de novo biosynthesis II 31948746.12 Pentose phosphate pathway (non-oxidative branch) 2090619.61
Adenosine deoxyribonucleotides de novo biosynthesis II 33696318.85 Adenosine deoxyribonucleotides de novo biosynthesis II 31948746.12 Superpathway of branched amino acid biosynthesis 2055294.89
Superpathway of L-isoleucine biosynthesis I 33623938.23 Superpathway of polyamine biosynthesis I 31898110.50 Fatty acid salvage 1993050.79
Phosphatidylglycerol biosynthesis II (non-plastidic) 32704959.97 Hexitol fermentation to lactate, formate, ethanol and acetate 31862604.60 Superpathway of phospholipid biosynthesis I (bacteria) 1991151.37
Phosphatidylglycerol biosynthesis I (plastidic) 32704959.97 dTDP-N-acetylthomosamine biosynthesis 31861896.87 Superpathway of sulfate assimilation and cysteine biosynthesis 1965566.64
Superpathway of adenosine nucleotides de novo biosynthesis II 32681382.85 Superpathway of L-isoleucine biosynthesis I 31801573.14 L-isoleucine biosynthesis III 1920492.83
Superpathway of polyamine biosynthesis I 32545588.40 Superpathway of L-methionine biosynthesis (transsulfuration) 31406712.49 Urate biosynthesis/inosine 5′-phosphate degradation 1915638.53
L-lysine biosynthesis III 32515917.24 Superpathway of adenosine nucleotides de novo biosynthesis II 30965700.29 8-amino-7-oxononanoate biosynthesis I 1899244.13
Superpathway of L-methionine biosynthesis (transsulfuration) 32509176.89 Phosphatidylglycerol biosynthesis II (non-plastidic) 30824987.37 Sulfate reduction I (assimilatory) 1899170.77
dTDP-N-acetylthomosamine biosynthesis 32439110.11 Phosphatidylglycerol biosynthesis I (plastidic) 30824987.37 Peptidoglycan maturation (meso-diaminopimelate containing) 1893845.34
Superpathway of L-phenylalanine biosynthesis 32293766.33 L-lysine biosynthesis III 30802845.50 Superpathway of adenosine nucleotides de novo biosynthesis I 1886912.51