ACN	acetonitrile
AF	annulus fibrosus
AGC	automatic gain control
ANOVA	analysis of variance
BrdU	bromodeoxyuridine
CEP	cartilaginous endplate
DAPI	4′,6-diamidino-2-phenylindole
DCFH-DA	2′,7′-dichlorodihydrofluorescein diacetate
dd	double-distilled
DMM	destabilization of the medial meniscus
DOX	doxorubicin
ECM	extracellular matrix
ERKs	extracellular signal-regulated kinases
FA	formic acid
FBS	fetal bovine serum
GAPDH	glyceraldehyde-3-phosphate dehydrogenase
IVD	intervertebral disc
JNKs	c-Jun N-terminal kinases
LC-HRMS	liquid chromatography-high resolution mass spectrometry
LD50	median lethal dose
LOD	limit of detection
LOQ	limit of quantification
MAPK	MAP kinase
MMP	matrix metalloproteinase
MRM	multiple reaction monitoring
MTT	methylthiazolyldiphenyl-tetrazolium bromide
NP	nucleus pulposus
OA	osteoarthritis
PBS	phosphate buffered saline
QC	quality control
ROS	reactive oxygen species
SIPS	stress-induced premature senescence
SPE	solid phase extraction
TGF-β1	transforming growth factor β1
TIMP1	tissue inhibitor of metalloproteinase 1