Figure 2 .

Examples of PTT gels. A, Fragment PTT3, transcripted and translated from cDNA of mouse; human (Normal); somatic-cell hybrids, 77-2/1 and P-MWH2A (HG only; lane 2 and 6, respectively); P-SHH1H; whole chromosome 16 (16 hybrid); and the radiation hybrid, Hy145.19 (PKD1 only). The product is only generated in PKD1-containing samples showing the specificity of this PTT assay. Analysis of PKD1 patient and normal cDNA by PTT assays, PTT1 (B) and PTT4 (C). The pedigree number and detected mutation are shown for each sample. Mutant polypeptides are seen as smaller products on the gel.