Table 2.

PTT and Sequencing Primers

Fragment	Sequence	AnnealingTemperature(°C)	Position(cDNA)	Size(bp)	Exon(s)
cDNA:
PTT 1	(F) GATGCCGAGAACCTCCTCG	63	1829–3869	2,041	8–15
	(R) TCATGTCCACGCTGAGTCCG
PTT 2	(F) GCCAACCACACCTATGCCTCG	62	3740–5431	1,692	15
	(R) GGCACTGAGGGTGACGCTTGT
PTT 3	(F) AGCGGCAAAGGCTTCTCGCTC	66	5246–7052	1,807	15
	(R) ACTCGCTCCCATCCAGCACC
PTT 4	(F) ACTGAGTACCGCTGGGAGGTGTATC	66	6758–7942	1,185	15–20
	(R) GGGCTCTGGGAGGGTGATG
PTT 5	(F) AAGACGCTGGTGCTGGATGAG	62	7448–8644	1,197	18–23
	(R) AGCCTCGGGGATGGAGAAG
cSeq 1	(F) TGAGTACCGCTGGGAGGTGTATC	62	6760–7336	577	15–17
	(R) TGCCTTGCAGGACACACACTC
cSeq 2	(F) TGTCCCTGAGGGTCCACACTG	62	8163–8681	519	22–23
	(R) GCACCACGTCACTGAGGTTGG
cSeq 3	(F) CTCAACGAGGAGCCCCTGAC	62	8516–9126	611	23–24
	(R) TCAGCACCCTGGAGTGACTCTG
cSeq 4	(F) CAGTCTACCTACACTCGGAGCCC	62	9039–9648	610	24–27
	(R) TCCACCCCATACAGCATGATG
Genomic:
GenSeq 1	(F) CCTGAGCTGCGGCCTCCG	64	3580–3799	220	1
	(R) CAGTTGACGCGGCAGGCG
GenSeq 2	(F) TGCGAGCCCCCCTGCCTC	66	3744–3923	180	1
	(R) AACCCGCCCACGCCCGCCCGTCC
GenSeq 3	(F) CTTGGGGATGCTGGCAATG	59	19810–20239	430	2–3
	(R) AACTGGGAGGGCAGAAGGG
GenSeq 4	(F) AGTGGGGGGCTGGCATAGAC	64	20371–21017	647	4–5
	(R) GCAAAGGAGGCACTGGAGGG
GenSeq 5	(F) CTTCTAGGTGAGGAGTATGTCGCC	64	20812–21593	782	5
	(R) AACGAGGGTGTCAACGGTCAG
GenSeq 6	(F) ACCGTTGACACCCTCGTTCC	60	21576–21855	280	6
	(R) TCTCTGCCCCAGTGCTTCAG
GenSeq 7	(F) CTGTGAGGGTGGGAGGATGG	64	22166–22787	622	7–8
	(R) GGAGGGCAGGTTGTAGAACGTG