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. 2025 Jul 31;12:1598991. doi: 10.3389/fnut.2025.1598991

Figure 2.

Western blot and bar graphs showing the effect of α-GML and staurosporine on protein and mRNA expression. Panel A displays protein bands for ZO-1, OCLN, and GAPDH. Panels B and C are bar graphs of ZO-1 and OCLN protein expression, while panels D and E show relative mRNA expression for ZO-1 and OCLN. Conditions vary under α-GML presence and different staurosporine concentrations. Statistical significance is noted with symbols.

α-GML promoted TJ expression through activation of PKC. (A) The effects on ZO-1 and OCLN protein expression of different concentrations of Staurosporine with or without α-GML. (B,C) Western blot quantification of ZO-1 and OCLN. (D,E) The mRNA expression of TJ proteins among different groups by qRT-PCR. Values are shown as means ± SEs, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001 vs. the DMSO group. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the α-GML treated group.