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. 2025 Jul 31;12:1598991. doi: 10.3389/fnut.2025.1598991

Figure 3.

Western blots and bar graphs illustrate protein and RNA expression. Panel A presents Western blots of ZO-1, OCLN, and GAPDH with differing SCH772984 concentrations. Bar graphs (B-E) quantify ZO-1 and OCLN protein and RNA expression, showing statistical significance with asterisks. Panel F depicts a bar graph for FITC dextran concentration. Panel G shows additional Western blots for ZO-1, OCLN, MAPK, p-MAPK, and GAPDH under various treatments. Bar graphs (H-K) quantify protein expressions from Panel G, indicating statistical significance.

α-GML promoted expression of TJ through PKC/MAPK signaling pathway. (A–C) The effects of different concentrations of SCH772984, with or without α-GML, on the expression of TJ proteins by western blot and quantification. (D,E) The mRNA expression of TJ proteins among different groups by qRT-PCR. (F) The results of FITC-dextran flux of cells treated with SCH772984 and α-GML. (G–K) Effects of Staurosporine and α-GML on the protein expression of phosphorylated and non-phosphorylated MAPK as well as TJ by western blot and quantification. Values are shown as means ± SEs, n = 3. ns, p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001 vs. the DMSO group. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the α-GML treated group.