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. 2025 Jul 31;12:1598991. doi: 10.3389/fnut.2025.1598991

Figure 5.

Western blot and bar graphs showing the effects of OE-ATF-2 and SCH772984 on protein and mRNA expressions. Panel A displays protein bands for ZO-1, OCLN, ATF-2, p-ATF-2, and GAPDH under different conditions. Panels B to E and G to H present bar graphs quantifying the relative expression levels of ZO-1, OCLN, ATF-2, p-ATF-2 proteins, and ZO-1 and ATF-2 mRNA. Individual bars indicate different combinations of OE-ATF-2 and SCH772984 presence, highlighting statistically significant differences with asterisks.

α-GML facilitated the expression of ZO-1 and OCLN through MAPK/ATF-2 signaling pathway. (A–E) Co-treated with SCH772984 and ATF-2 overexpression plasmid to detect TJ and p-ATF-2 expression by western blot assay and quantification. (F–H) The mRNA expression of TJ proteins among different groups by qRT-PCR. Values are shown as means ± SEs, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001 vs. the DMSO group.