Fig. 5.

Blocking STAT3 SH2 domain increases mortality in C8-D1A astrocytic cells. Cells were pretreated with 0, 3, or 30 µM C188-9, followed by co-treatment with 0 or 10 µM MeHg. Cytotoxicity (LDH release) was measured using LDH at 3 h (A), 6 h (B), and 24 h (C) of MeHg addition. Cell viability was assessed using MTT at 3 h (D), 6 h (E), and 24 h (F) of MeHg addition. Data are presented as mean ± SD. Statistical significance was determined using two-way ANOVA followed by Bonferroni’s post-hoc analysis. When data did not meet the assumptions of normality, a logarithmic or square root transformation was employed before conducting the two-way ANOVA. p < 0.05 was considered statistically significant. * denotes a significant difference