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. 2025 Aug 13;15:29738. doi: 10.1038/s41598-025-97186-x

Fig. 4.

Fig. 4

Electrophysiological effect of Aβ exposure on co-culture glutamatergic neurons. (a) Timeline of Aβ exposure experiment steps. (b) Bar graph of WMFR in microchannels of chronic Aβ exposure in time respectively. The data were analyzed using a two-way ANOVA to evaluate the effects of 1µ2 and 2µ3 and their interaction in time. Fisher’s Least Significant Difference (LSD) test was used as a post-hoc analysis to compare specific group means. * p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001 and **** p-value < 0.001. Error bar = SD. MFR: Mean firing rate, WMFR: weighted mean firing rate.