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. 2025 Aug 14;16(4):e70045. doi: 10.1002/jcsm.70045

FIGURE 5.

FIGURE 5

Functional characterization of hSkMOs. (A) Spontaneous contraction and (B) calcium imaging with Fluo‐4 AM for visualizing calcium influx of Day 100 hSkMOs. Scale bar = 50 μm. (C) Representative trace of the spontaneous postsynaptic potential (PSP) response obtained from the section of the organoid with muscle fibres (top). Enlarged PSP response observed (bottom left). Their amplitude and frequency (bottom right) from individual muscle fibres targeted (mean ± SEM; n = 8). (D) Representative trace graph depicting spontaneous contractions in mature hSkMOs, and their pharmacological modulation by acetylcholine (ACh) and curare. (E) Quantitative analysis of contraction dynamics in control, ACh‐treated, and curare‐treated hSkMO (mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; n = 3). (F) ACh and curare affect the postsynaptic potential response from the section of hSkMOs. Traces obtained from three independent cells presented and responses are marked with asterisks.