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. Author manuscript; available in PMC: 2025 Aug 15.
Published in final edited form as: Sci Transl Med. 2024 Nov 20;16(774):eadl2184. doi: 10.1126/scitranslmed.adl2184

Fig. 2. Vagal regulation of colitis in mice is IL-10-independent and mediated by the spleen.

Fig. 2.

Time course of (A) DAI, (B) body weight, (C) intestinal permeability, and (D) colon IL-6 and TNF-α concentrations in (a to d) IL10-KO (IL10KO) mice and their wild-type (WT) littermates at day 7 after 5-day DSS challenge. Time course of (E) DAI, (F) body weight, (G) intestinal permeability, and (H) colon IL-6 and TNF-α concentrations in (E to H) IL10KO at day 7 after receiving sham or vagal stimulation (VS) immediately followed by 5-day DSS challenge. Time course of (I) DAI, (J) body weight, (K) intestinal permeability, and (L) colon IL-6 and TNF-α concentrations in (I to L) splenectomized (SPX) or sham mice at day 7 after 5-day DSS challenge. Mice were splenectomized 5 days before the DSS, sham, or VS treatment. Time course of (M) DAI, (N) body weight, (O) intestinal permeability, and (P) colon IL-6 and TNF-α concentrations in (M to P) splenectomized (SPX) at day 7 after receiving sham or vagal stimulation (VS) immediately followed by 5-day DSS challenge. *P<0.05 vs Control, #P<0.05 vs DSS (n=5, One-way ANOVA with Bonferroni’s post-hoc test). Graphs represent the mean ± SEM of experiments repeated twice on different dates.