Fig. 4. Nicotine improves colitis outcomes in mice through α7nAChRs.

Time course of (A) DAI, (B) intestinal bleeding, and (C) body weight, and (D) colon length, intestinal permeability, and (E) colon immune cytokines in (A to E) wild-type mice at day 7 after 5-day DSS challenge with 3-day nicotine treatment or vehicle. Timecourse of (F) DAI, (G) intestinal bleeding, and (H) body weight, and (I) colon length, intestinal permeability, and (J) colon immune cytokines in (F to J) Chrna7-KO (α7KO) mice at day 7 after 5-day DSS challenge with 3-day nicotine treatment or vehicle. *P<0.05 vs Control, #P<0.05 vs DSS (n=5, One-way ANOVA with Bonferroni’s post-hoc test). Graphs represent the mean ± SEM of experiments repeated twice on different dates.