FIGURE 2.

Volume analysis of hTERT‐RPE‐1 cells by confocal light microscopy. (A) Single slice from an acquired confocal Z‐stack of a fixed hTERT‐RPE‐1 cell in interphase. The cell membrane and the cytoplasm are shown in red, the nucleus in cyan. (B) Single slice showing only the nucleus in the confocal Z‐stack as illustrated in A. (C) Single slice showing only the membrane and cytoplasm in the confocal Z‐stack as shown in (A). (D–F) Volumetric view of the 3D‐segmented cell, nucleus and cytoplasm as shown in A–C. Scale bars, 5 µm.