Figure 2.

Intra-arterial infusion of acidified malonate is protective against stroke IRI following tMCAO, measured at 2 h. a: The occlusion suture is first inserted into the internal carotid artery (CA) and advanced to the MCA to bring about MCAO, as verified by measuring cerebral blood flow (CBF) by laser Doppler flowmetry. Shortly prior to reperfusion, the infusion catheter is then inserted into the CA on top of the occlusion suture and guided to the region of occlusion/ischemia. Withdrawal of the occlusion suture from the MCA back to the CA initiates reperfusion. The infusion catheter is used to infuse 50 µL saline or drug from 2 min before to 2 min after the onset of reperfusion at the site of ischemia. B: Representative TTC-stained brain slices assessed 2 h after reperfusion with either saline or indicated doses of aDSM. C: Dose response of brain infarct volume to aDSM assessed by TTC staining 2 h after reperfusion. Data are mean infarct volume ± SEM, n = 5–9. Statistics: One-way ANOVA with Tukey’s post hoc test.