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. 2001 Jan 10;68(2):397–409. doi: 10.1086/318197

Figure 4.

Figure 4

Immunoblotting of mitochondria from fibroblast cultures. Mitochondria (100 μg) were prepared from skin fibroblast cultures, were subjected to PAGE separation, and were transferred to nitrocellulose. Parallel prepared blots were then probed with antibodies to beef heart COX (top), SURF1 (middle), and COX7RP (7aRP) (bottom). The anti-COX antibody recognizes subunits II, IV, Vb, and VIa of the human COX complex. Lane 1, Control. Lanes 2, 3, and 5, Patients with LS and SURF1 mutation. Lane 4, Patients with LS COX deficiency and normal SURF1. Lanes 6, 7, and 8, Patients with LSFC-COX.