Table 1.

Primers and DHPLC Conditions for Mutational Analysis of Human ATM

	Primer		Length of Sequence(bp)			DHPLCConditions
ATMExon	Forward(5′→3′)	Reverse(5′→3′)	Coding	Noncodinga	Total	Temperature(°C)	Gradient(% ofBuffer B)
Promotor	GCCCAGAACCTCCGAATGACG	CGACTTAGCGTTTGCGGCTCG	0	493	535	63	59–64
1A	AATGTTTTGGGGCAGTGTTT	AGGAAAGATGGAGTGAGGAGAG	0	370	412	63	58–63
1B	GACTCCTCCCTCTCCTCACTC	CCATCTGGAAGGCTTCTACC	0	515	556	60	60–65
1B, 2	TGCAAACTCAGCCTGAGACT	CATGCCATTCTTTTCTAGTGC	0	491	532	59	54–60
3	TGAACCTTGAGTGGAACCTA	CTCTGAGGAAACAAAAACACA	0	294	335	57	53–58
3	TTGGCACCAGTTAGTTCAGA	CAGAAGATGCTCATTCACTGA	0	260	300	54	55–60
4, 5	TACACATTTTTTCACACCTCTTTC	AGGCATAATGATATATAGGAAGCA	185	263	496	50, 55	60–66, 58–64
6	ATTGGTCTTGTAGGAGTTAGGC	AAAAACTCACGCGACAGTAATC	146	202	392	54	58–64
7	TTGCCAATTTCTTCTCTACAAAAG	TTAAGGGTCAGTTCGATAACCATA	165	241	454	55	54–60
8	CTTTTTCTGTATGGGATTATGGA	TTCTGTTATGATGGATCAATGTTT	166	177	390	50, 55	57–63, 51–56
9	CAGCATACCACTTCATAACTG	TCATATCCTCCTAAAGAACAC	239	131	412	54	56–61
10	CTAGCAGTGTAAACAGAGTA	ATAGGCTTTTTGTGAGAACA	164	121	325	54	55–60
11	GCAACAACAGCGAAACTCTG	GAATGAGAAAATGGTAACACTT	170	139	350	53, 56	56–61, 53–58
12	TGTGATGGAATAGTTTTCAA	AGTAACAAACTATGAAAATGA	372	98	511	57	56–62
13	CAATAGCTTGCTTTTCACAAT	TGGCATCAAATAAGTGGAGAG	195	92	335	57	54–59
14	GATGAAAGCAATTTTAATCTAGGA	TCAGTTTTTCTCATTGGCACTT	96	340	482	54	56–62
15	CATATAAGGCAAAGCATTAG	AGTTTACCAAAGTTGAATCATA	226	72	340	55	53–59
16	GTTGTTTTTAGAGCTATCCAGGA	CTGCATCATGTACCCCAGAAC	126	332	502	53, 57	58–63, 55–61
17	CGTGGAACTTCTAAAAACATTTC	CAAAACAGTAACACCAACCAGT	126	346	517	54, 59	59–65, 54–59
18	AGCTCACTTATCTTTAGGGTCAA	GGCCTTAATTTCCACATTTGT	90	329	463	54	57–62
19	CTCCTGCAAGAAGCCATCT	AAGAAATCCCAAGTAGTAAAT	172	38	250	57	53–58
20	CTTTTTTTTGTGAAGAGGAGGA	TTTCAATTCTTCAAAGACACCA	200	156	400	51, 58	58–64, 52–58
21	TTTTTCCCTCCTACCATCTT	CTTAACAGAACACATCAGTTATT	83	78	173	55	50–55
22	TAAAATAACTGATGTGTTCTGTT	CAAAACTTGCATTCGTATC	156	150	286	56	54–60
23, 24	TAGCACAGAAAGACATATTGGAAG	TGTAAGACATTCTACTGCCATCTG	207	236	491	55	56–61
25	AGCTGCTGGTCTGAACCTCTTTA	TTGCTATGATTTGACCCATTGTG	118	324	488	54, 57	56–60, 51–57
26	TGGAGTTCAGTTGGGATTTTA	TTCACAGTGACCTAAGGAAGC	174	87	303	51, 55	58–64, 56–61
27	CTTAACACATTGACTTTTTGG	GTATGTGTGTTGCTGGTGAG	170	78	288	54	54–59
28	TGCTGATGGTATTAAAACAGTTT	GGTTGGCTATGCTAGATAATGAT	247	192	485	51, 56	58–64, 54–60
29	GAGCTGTCTTGACGTTCACAG	TTAAAAAGAGTGATGTCTATAA	116	131	290	53, 57	56–61, 51–57
30	TTAAAACGATGACTGTATT	GAAGGAATGTTCTATTATTA	127	40	207	56	51–56
31	CTGAAAATTAAATAAATTGGCAAT	AAAACAGGAAGAACAGGATAGAAA	200	227	475	54	56–61
32	CTTACTGGTTGTTGTTGTTTT	CCATTTTGAAGATGAGTCAG	175	26	241	57	52–58
33	GTTTTGTTGGCTTACTTTA	GAGCATTACAGATTTTTGAA	165	99	304	53	53–59
34	GTGTTAAAAGCAAGTTACATT	AAGAAACAGGTAGAAATAGC	133	33	207	56	50–55
35	GCAATTATAAACAAAAAGTGT	TATATGTGATCCGCAGTTGAC	96	78	215	57	53–58
36	CAGCATTATAGTTTTGAAAT	GTGTGAAGTATCATTCTCCA	172	114	326	55	54–60
37	TGGTGTACTTGATAGGCATTT	GACCCACAGCAAACAGAACTG	142	63	245	57	52–57
38	AACCTAATTTTTCTGCTGCCTAA	GAGTGGGGGTGATATTATGTGA	177	279	501	55	58–64
39	GCAGTATGTTGAGTTTATGGCA	GCAACTGTTGGCAACTTTTAT	178	260	481	53, 57	58–64, 54–60
40	ATAAACAAGAAGGAAGAAGGT	ACGTAAGAAGCAACACTCATT	88	56	184	58	51–56
41	CAACATGCTTTTATTTTGATA	TATATACCCTTATTGAGACAA	156	90	288	55	53–59
42	TCTCATTAAAAGAGGTGTTCT	TGAGATAAATACTGTCATAAA	88	34	164	56	(49) 51–57
43, 44	AAATTTGCTAAATTTATAGACCGA	AGTGATGGCTTTACCAAATCTGG	192	152	391	57	55–60
45	TTTGCTGTTTTTTTCTCTGGT	CAGTTGTTGTTTAGAATGAGGAGA	149	91	285	56	53–59
46	ATTTTGTCCTTTGGTGAAGCTA	CAAGTTTTCAGAAAAGAAGCCA	105	109	258	53	55–60
47	GCAAAGCCTATGATGAGAACTC	CAGAAAAGCTGCACTTTAGGAT	120	103	267	53, 57	55–61, 50–55
48	TCTTGTCACTACAAAAGTTCCTTT	TCTTTTTCCCTCAGGCTTTC	235	165	444	51, 56	61–67, 56–61
49	CCTCAATGAATGGTAGTTGCT	GTAAAACACTAATCCAGCCAATA	168	87	299	53, 58	57–63, 51–56
50	CCAAAGCAGATGAGGAAAAAC	TCTTGATGAAAAGATGAAGCATAT	114	165	325	56	54–59
51	GTGTATTACCTTAATTTGAGTG	CCAAAAGACCAAGATAATCT	218	191	451	55	61–67
52	TCCTTAGAAGTTTGCTTTTTTC	CTGGACCAAGTGCTAGGAATA	208	199	450	57	57–62
53	CTAGAGTACCCATTAGAAAGACCT	GTGTATGCCTGCATGTGTGA	114	346	504	50, 55	64–69, 59–64
54	CTCTGCCTTTTTCTCACACAT	CCTTGAACCGATTTTAGATGG	159	218	419	56	54–60
55	GGGTAGTTCCTTATGTAATGT	GTAACACAGCAAGAAAGTAACGT	139	42	225	56	50–56
56	GACCTTCAATGCTGTTCCTCAGT	AGGTTGAAACATATGAAATTTGCC	83	135	265	54	52–58
57	ACCCGGCCTAAAGTTGTAGT	AATGGAGAAAAGCCTGGTTC	141	278	459	57	53–59
58	TTTGCTATTCTCAGATGACTCT	ATGTTtTTGGTGAACTAACAGAAG	117	69	232	56	51–56
59	GCTGAATGATCATCAAATGCTCT	ATAATATCTGACAGCTGTCAGCT	150	99	295	52, 57	60–66, 54–60
60	TTTATTGCCCCTATATCTGTCAT	AAAAAGTGCTGAATCAAACAAA	166	134	345	55	56–61
61	CAACATGGCCGGTTATGC	CACTCAGTACCCCAGGCAGA	87	305	430	54	57–63
62	GTGGTTTCTTGCCTTTGTAAAGTT	AATCCTCCCACTTCAGCCTCTT	115	162	323	58	58–63
63	TAGGCTCAGCATACTACACAT	GACGAGATACACAGTCTACCT	64	117	223	55	51–56
64, 65	GGCTTATTTGTATGATACTGGTTC	CTAAAGGCTGAATGAAAGGGTA	318	191	555	53, 58	60–65, 55–61
65	TACCCTTTCATTCAGCCTTTAG	TTTTTTTTTTGAGATGGAGTTTC	0	470	515	56, 61	64–69, 59–64
65	TCTCAAAAAAAAAAAAAAAAAACA	TGGGAATATGACATAAACAGACA	0	538	586	53	60–65
65	AGGCTTTATCTATGGGAATCTT	AGAGGTGAATATGTGAGCTGAT	0	370	414	59	56–61
65	CTCATTTTTGACCGTAAGGA	AAGTTCTGGAGATTGGTTTTAG	0	411	453	56	59–64
65	CCTCCCCTAAAACCAATCT	AGTTATTTCTCCTAGGCTTGTG	0	417	459	54	58–63
65	ATGGCTTTGAAAAGTTTATCA	TAGGGTGGGAAAGCTATTATC	0	579	621	56	56–61
65	ATGAAAACCAAATAGTGAAGC	TGGCACACAGAACACACAA	0	467	508	55	59–64

^aNoncoding length does not include primer sequence.