Table 1.
Statistical analysis using a repeated measures 2-way analysis of variance of mechanical sensitivity and self-administration presented in Figure 1.
| F (DFn, DFd) | P | |||
|---|---|---|---|---|
| Figure 1B von frey (VF) | Combined sexes | Time | F (4, 148) = 22.98 | P < 0.0001**** |
| Surgery condition | F (1, 148) = 71.35 | P < 0.0001**** | ||
| Time × surgery | F (4, 148) = 8.045 | P < 0.0001**** | ||
| Males | Time | F (4, 84) = 17.79 | P < 0.0001**** | |
| Surgery condition | F (1, 84) = 35.75 | P < 0.0001**** | ||
| Time × surgery | F (4, 84) = 4.769 | P = 0.0016** | ||
| Females | Time | F (4, 55) = 7.918 | P < 0.0001**** | |
| Surgery condition | F (1, 55) = 65.62 | P < 0.0001**** | ||
| Time × surgery | F (4, 55) = 6.916 | P = 0.0001**** | ||
| Figure 1C Both sexes |
Reinforcers | Time | F (4, 116) = 20.60 | P < 0.0001**** |
| Pain | F (1, 29) = 4.068 | P = 0.0531 | ||
| Time × pain | F (4, 116) = 0.6599 | P = 0.6211 | ||
| Males | Reinforcers | Time | F (4, 64) = 7.772 | P < 0.0001**** |
| Pain | F (1, 16) = 2.794 | P = 0.1140 | ||
| Time × pain | F (4, 64) = 1.338 | P = 0.2655 | ||
| Females | Reinforcers | Time | F (4, 44) = 19.90 | P < 0.0001**** |
| Pain | F (1, 11) = 1.174 | P = 0.3018 | ||
| Time × pain | F (4, 44) = 0.4512 | P = 0.7709 | ||
| Figure 1C | Time to achieve max reinforcers | Time | F (3.261, 94.57) = 6.866 | P = 0.0002*** |
| Pain | F (1, 30) = 4.444 | P = 0.0435* | ||
| Time × pain | F (4, 116) = 0.6698 | P = 0.6142 | ||
| Figure 1c | Survival curve | X2 = 9.015 | P = 0.0027** | |
| Figure 1D Both sexes |
Nose pokes | Time | F (4, 145) = 2.564 | P = 0.0408* |
| Nose poke | F (1, 145) = 56.11 | P < 0.0001**** | ||
| Pain | F (1, 145) = 17.09 | P < 0.0001**** | ||
| Time × nose poke | F (4, 145) = 3.005 | P = 0.0204* | ||
| Time × pain | F (4, 145) = 0.8918 | P = 0.4706 | ||
| Nose poke × pain | F (1, 145) = 1.184 | P = 0.2784 | ||
| Time × nose poke × pain | F (4, 145) = 0.2866 | P = 0.8863 | ||
| Figure 1D Males |
Nose pokes | Time | F (4, 145) = 2.420 | P = 0.0511 |
| Nose poke | F (1, 80) = 50.88 | P < 0.0001**** | ||
| Pain | F (1, 145) = 11.79 | P = 0.0008*** | ||
| Time × nose poke | F (4, 80) = 1.410 | P = 0.2383 | ||
| Time × pain | F (4, 145) = 1.641 | P = 0.1670 | ||
| Nose poke × pain | F (1, 80) = 0.2959 | P = 0.5880 | ||
| Time × nose poke × pain | F (4, 80) = 1.356 | P = 0.2566 | ||
| Figure 1D Females |
Nose pokes | Time | F (4, 145) = 2.566 | P = 0.0407* |
| Nose poke | F (1, 54) = 22.80 | P = 0.0002*** | ||
| Pain | F (1, 145) = 12.77 | P = 0.0005*** | ||
| Time × nose poke | F (4, 54) = 3.607 | P = 0.0112* | ||
| Time × pain | F (4, 145) = 0.4045 | P = 0.8052 | ||
| Nose poke × pain | F (1, 54) = 0.7256 | P = 0.3981 | ||
| Time × nose poke × pain | F (4, 54) = 1.166 | P = 0.3361 |
P values are provided for each of the factors (time, surgical condition, and if there was an interaction between time and surgical condition). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.