Table 5.

Statistical analysis using repeated measures 2-way analysis of variance of remifentanil during acquisition of opioid intravenous self-administration, where data are presented in Figure 5.

Figure 5			F (DFn, DFd)	P
Remifentanil IVSA
Figure 5B	Reinforcers	Time	F (2.602, 54.12) = 4.781	P = 0.0071**
		Pain	F (1, 22) = 1.278	P = 0.2705
		Time × pain	F (5, 104) = 0.5308	P = 0.7525
Figure 5C	Nose pokes	Time	F (5.000, 130.0) = 4.848	P = 0.0004***
		Nose poke	F (0.4568, 11.88) = 12.55	P = 0.0120*
		Pain	F (1, 26) = 0.01537	P = 0.9023
		Time × nose poke	F (3.056, 42.17) = 31.15	P < 0.0001****
		Time × pain	F (5, 130) = 0.6918	P = 0.6305
		Nose poke × pain	F (1, 26) = 0.2111	P = 0.6198
		Time × nose poke × pain	F (5, 69) = 0.6418	P = 0.6686
Figure 5C	% accuracy	Time	F (3.402, 68.05) = 38.54	P < 0.0001****
		Pain	F (1, 22) = 0.1057	0.7482
		Time × pain	F (5, 100) = 0.1788	0.9700
Figure 5E (before reversal)	Day 1	Time × pain	F (120, 2040) = 2.275	P < 0.0001****
		Time	F (1.595, 27.12) = 66.34	P < 0.0001****
		Pain	F (1, 17) = 2.187	P = 0.1575
	AUC		t = 1.479, df = 17	P = 0.1575
	Day 3	Time × pain	F (120, 2040) = 0.06291	P > 0.9999
		Time	F (2.161, 36.74) = 116.4	P < 0.0001****
		Pain	F (1, 17) = 0.1357	P = 0.7171
	AUC		t = 0.3684, df = 17	P = 0.7171
Figure 5E (after reversal)	Day 1	Time × pain	F (120, 2040) = 1.371	P = 0.0058**
		Time	F (1.515, 25.76) = 38.84	P < 0.0001****
		Pain	F (1, 17) = 0.4521	P = 0.5104
	AUC		t = 0.6724, df = 17	P = 0.5104
	Day 3	Time × pain	F (120, 2040) = 0.5605	P > 0.9999
		Time	F (1.400, 23.80) = 72.10	P < 0.0001****
		Pain	F (1, 17) = 0.6479	P = 0.4320
	AUC		t = 0.8049, df = 17	P = 0.4320

An unpaired t test was used for area under the curve (AUC) data. P values are provided for each of the factors (time, pain, and appropriate interactions) for active and inactive nose pokes, and number of reinforcers earned. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

AUC, area under the curve; IVSA, intravenous self-administration.