Figure 3.

Southern analysis of EC hybrids and their parental A9 clones. A, Lanes grouped such that each A9 clone is followed by its derivative EC clone(s). Clonal DNAs were cleaved by EcoRI plus EagI. Fragments between 2.8 and 5.2 kb (see control lane C) represent unmethylated alleles. Demethylation of previously methylated EagI sites is evident in GZEC24.16, MLEC30.1, and MLEC30.2. Repeat sizes of all clones are given in table 2. B and C, MLEC30.1, subcloned by dilution plating, and subclonal DNAs, analyzed on EcoRI-plus-EagI (B) and PstI blots (C). Results for 10 subclones are indicated by numbers above the lanes. Blurred and smeared signals consistent with mitotic instability of subcloned expansions occurred as a function of repeat size.