BMDM-CM

Reagent	Final concentration	Amount
RPMI 1640 medium	N/A	388.5 mL
FBS	10%	50 mL
Penicillin-Streptomycin	1%	5 mL
L929 conditional medium	10%	50 mL
Antibiotic-Antimycotic	1%	5 mL
β-Mercaptoethanol	55 μM	0.5 mL
Sodium Pyruvate	1 mM	0.5 mL
GlutaMax	2 mM	0.5 mL
Total	N/A	500 mL

Store at 4°C for up to 4 weeks.