TABLE 2.
Detection of E. bieneusi spores in stool of rhesus macaque
Detection by PCR (first and nested PCR) | No. of samples with result
|
||||||||
---|---|---|---|---|---|---|---|---|---|
All samples (n = 38) | Detection by MAb 2G4a,b
|
Detection by rabbit antibodya,b
|
|||||||
+ | ++ | +++ | ++++ | + | ++ | +++ | ++++ | ||
Positive by both PCR | 22 | 3 | 4 | 7 | 8 | 2 | 4 | 5 | 11 |
Negative by first PCR but positive by nested PCR | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Negative by both PCR | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Spores were counted in 10 microscopic fields at a magnification of ×400.
+, 40 to 100 spores; ++, 101 to 400 spores; +++, 401 to 1,000 spores; ++++, >1,000 spores.