FIG. 4.

Inhibition of JAK-STAT signaling in response to IFN is associated with inhibition of Jak1 and Tyk2 phosphorylation. Vero cells were infected with LGTV strain TP21 for 48 h and treated with IFN-α or IFN-γ for 15 min. The cell lysates were examined by immunoprecipitation using antibodies to Tyr1022/Tyr1023-phosphorylated Jak1 (pY-Jak1) or Tyr1054/1055-phosphorylated Tyk2 (pY-Tyk2). The immunoprecipitates were analyzed by Western blotting using antibodies that recognize Jak1 or Tyk2. Whole-cell lysates were also examined by Western blotting to determine steady-state levels of Jak1 and Tyk2.