FIG. 6.

Replication of VZV ORF66 mutants in skin xenografts in SCID-hu mice. Skin tissue was infected with pOka and pOka66S grown in HEL fibroblasts. Implants were harvested after 14, 21, and 28 days, and virus was titrated on melanoma cell monolayers by infectious focus assay. Each time point represents the mean number of plaques from three to four implants. Implants that did not contain infectious virus were excluded from the average. Inocula (shown as boxes on the y axis) are given as infectious foci/100 μl, which represents the amount used to infect each implant. One asterisk indicates that titers were significantly different from pOka with a P value of <0.01 at the same time point, while two asterisks indicate a difference with a P value of <0.001.