FIG.6.

Budding capsids in RER cisternae of Vero 2-2 cells at 12 h postinfection (A, C, and D) and of HeLa cells at 16 h postinfection (B and E) with HSV-1. (A) RER membrane continuous with the outer nuclear membrane (o) is associated with Golgi membranes surrounding a virion (inset). A cytoplasmic capsid is in an early phase of budding at the site where RER and Golgi membranes are associated. (B to D) Capsids in initial (B), early (C), and late (D) phases of budding into RER cisternae, for which the membrane situated close to the nucleus (n) has only a few ribosomes (r) in panel C. Note that all membrane capsid buds are thickened by a dense substance. (E) Golgi complex with narrow cisternae filled with a dense substance, probably tegument. Note the continua between Golgi and RER membranes (arrows). Bars, 100 nm.