TABLE 1.

Number of virus particles during envelopment or of impaired nuclear pores in 20 cellular profiles chosen at random from ultrathin sections of Vero cells incubated for 12 or 15 h after infection with HSV-1 at an MOI of 1

Pathwaya	Phenotypeb	No. of particles with phenotype or indicated value
		8 h	12 h	15 h
NE	Budding IM		1	7
NE/CE	Virions in PNS		3	81
NE/CE	Virions in RER		0	18
NE/CE	Virions in Golgi cisternae		6	2
NE/CE	Virions in P vacuoles		47	90
	Number of P vacuoles		19	25
CE	Cytoplasmic capsids		0	61
CE	Budding at OM		1	16
CE	Budding at RER		0	1
CE	Budding at Golgi cisternae		15	2
CE	Budding at Golgi fragments		2	5
CE	Budding at P vacuoles		14	21
CE	W vacuoles		41	16
CE	Impaired nuclear pores		1	3
	Ratio of Nup153 dislocation to ICP4 particles	1

^aNE, nuclear envelopment; CE, cytoplasmic envelopment.

^bIM and OM, inner and outer nuclear membranes, respectively; PNS, perinuclear space; P vacuoles, large vacuoles derived by packaging (Fig. 4B); W vacuoles, small vacuoles with one virion and an electron-dense substance between the envelope and the vacuolar membrane (Fig. 5D).