TABLE 1.

Viral DNA copy number of wild type or E4 mutant HPV16 genomes

Genome	Presence of extrachromosomal viral DNAa	Plasmid copy no.b,d	Relative plasmid copy no.c,d
WT	Y	8.4 ± 3.5	1
16st10	N	1.0 ± 0.2*	0.1*
16st15	Y	11 ± 2.5	1.3
16st20	Y	80 ± 21*	7.4*
16st32	Y	31 ± 14*	4.9*
16st67	Y	4.4 ± 2.3	0.5
16st84	Y	9.6 ± 1.5	1.2
PSS/WT	N	1.3 ± 0.3*	0.2*
PSS/20	N	1.6 ± 0.3*	0.2*

^aY, extrachromosomal viral DNA was detected; N, extrachromosomal DNA was not detected (see Fig. 2).

^bViral DNA copy number in each NIKS cell population was estimated based on the intensity of copy number standard. Each value is the average copy number of the wild-type or E4 mutant HPV16 genome, based upon analyses of multiple (n > 10 per genotype) independent cell populations generated from a representative set of transfections.

^cThe copy number for wild-type HPV16 populations was set at 1.

^d*, copy number values for E4 mutant HPV16 genomes that were statistically significant from that of the wild-type HPV16 genome, based on the two-sided Wilcoxon rank sum test.