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. 2005 Oct;79(20):13082–13093. doi: 10.1128/JVI.79.20.13082-13093.2005

FIG. 4.

FIG. 4.

The C terminus of VP22 is essential for assembly into the virion. Extracellular virions were prepared from cells infected with HSV-1 expressing GFP-tagged VP22 consisting of the full-length protein (residues 1-301), residues 1-212, or residues 1-165. Equivalent numbers of virions were analyzed by SDS-PAGE, followed by (A and B) Coomassie blue staining or (C) Western blotting with anti-VP22 or anti-VP16 antibodies. Asterisks denote the full-length GFP-VP22 band present in the virion profile. The values on the left of each panel are molecular sizes in kilodaltons.