FIG. 2.

Western blot assay of truncated pORF2 expressed in Sf9 and Tn5 cells. Eight recombinant baculoviruses, Ac[n111], Ac[n111c52], Ac[n111c58], Ac[n111c59], Ac[n111c60], Ac[n111c64], Ac[n111c72], and Ac[c52], were used to infect the insect cells. Ten microliters of the culture medium (right column) and 5 μl of the cell lysate (left column) were separated by 10% SDS-PAGE, and HEV-specific proteins were detected by Western blot analysis using the serum of a patient with acute hepatitis E. M, molecular weight markers; W, wild-type baculovirus-infected cells.