FIG. 1.

Schematic representation of the constructs used in the present study. Shown are the common elements contained in the binary vectors' right and left borders of transfer DNA (RB and LB, respectively), the promoter of cauliflower mosaic virus (CaMV) 35S (P35S or 35S), the terminators of CaMV35S or nopaline synthase (T35S and Tnos, respectively), and genes inserted into the multicloning sites between promoters and terminators. In the diagram of pE3-S10stop, the arrow with the text “stop” above it indicates the site of the termination codon. pJawohl8-IR GFP contains inverted repeats of gfp sequences separated by an intron (Int.) from the Arabidopsis thaliana WRKY gene. In the PVX chimeras, genes are inserted between two PVX coat protein (CP) promoters shown as short vertical bars. The deletion and frameshift mutants of RDV S10 (ΔS10) and PVX 25K (Δ25K) contained in the PVX chimeras are shown as interrupted rectangles.