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. 2005 Oct;79(20):13018–13027. doi: 10.1128/JVI.79.20.13018-13027.2005

FIG. 4.

FIG. 4.

Distantly expressed RDV Pns10 blocks or inactivates systemic silencing signals. (A) Schematics showing infiltration of the indicated DNAs into different parts of a leaf or different leaves on a plant of N. benthamiana line 16c. (B) Images 1 and 2 show the different effects of Pns10 on systemic GFP silencing when the plants are infiltrated as depicted in images 1 and 2 of panel A. Letters B and T in parentheses denote the base and tip portions of the leaf infiltrated with the indicated DNA, respectively. (C) Northern blot analysis of both GFP mRNA and siRNA from samples shown in Fig. 3 and panel B. Lanes 1, 2, and 3 correspond to samples shown in panels 2 and 3 of Fig. 3 and image 1 of panel B, respectively. Lanes 4 and 5 correspond to samples shown in panel 1 of Fig. 3 and image 2 of panel B, respectively. All RNAs were extracted from plants at 15 dpi.