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. 2005 Oct;79(20):13018–13027. doi: 10.1128/JVI.79.20.13018-13027.2005

FIG. 7.

FIG. 7.

RDV Pns10 enhances pathogenicity of chimeric PXV. (A) Image 1 shows a leaf from a mock-infected plant. Leaves infected with PVX (image 2) or PVXΔS10 (image 3) show mild disease symptoms as a few scattered chlorotic speckles, whereas leaves infected with PSV-S10 show extensive necrotic mottles (image 4). (B) RNA gel blot analysis of accumulation of PVX genomic (gRNA) and subgenomic mRNAs (sgRNA1 to sgRNA3) at 9 and 20 dpi. Equal loading of total RNA was verified by spectrometric measurement and ethidium bromide staining of 28S RNA as indicated at the bottom of the panel.