FIG. 6.

Blocking HSPG interactions reduces HTLV-1 virion binding to T cells. Concentrated HTLV-1 virions were incubated for 30 min in the presence of 0, 10, or 25 μg/ml of soluble heparin and then incubated with SupT1 (A) or activated CD4⁺ T cells isolated from adult peripheral blood (B), and the amount of virion binding was determined as described in Materials and Methods. (C) MOLT4 cells and activated CD4⁺ T cells isolated from adult peripheral blood were cultured either in RPMI containing 30 mM sodium chlorate or in RPMI alone for 3 days. The cells were then analyzed for virus binding. The data shown are from a representative experiment out of five (A and B) or three (C) performed.